Virus:Article Title: OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Calnexin Invitrogen Cat# PA5-34754; RRID: AB_2552106 Chromogranin A Proteintech Cat# 10529-1-AP; RRID: AB_2081122 GolgiB1 Bioss Cat# BS-13356R Insulin Dako Cat# A0564; RRID: AB_10013624 IP3R1 Abcam Cat# ab264281 OSBP Invitrogen Cat# PA5-30110; RRID: AB_2547584 PI4K2A Santa Cruz Biotechnology Cat# sc-390026 Rab3 Synaptic Systems Cat# 107 111 VAPA Atlas Antibodies Cat# HPA009174; RRID: AB_1080549 VAPB Atlas Antibodies Cat# HPA013144; RRID: AB_1858717 b-actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 Bacterial and virus strains Ad-CMV-GB-Rab3 Vector Biolabs Lot# 20220125T#2 Ad-CMV-RA-Sec61b Vector Biolabs Lot# 20220125T#3 Chemicals, peptides, and recombinant proteins BCECF Life technologies Cat# B1170 Cal-520-AM AAT Bioquest Cat# 21130 Cal-590-AM AAT Bioquest Cat# 20510 OSW-1 Cayman Chemical Cat# 30310 TopFluor Cholesterol (23- [dipyrrometheneboron difluoride]24-norcholesterol] Avanti Polar Lipids, Inc. Cat# 810255 Filipin III Cayman Chemical Cat# 70440 JFX650 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JF549 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JFX646 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A Carbamoyl chloride Tocris Cat# 2810 Phenylarsine oxide Sigma Aldrich Cat# P3075 PIK 93 Sigma Aldrich Cat# SML0546 PI4KI MedChemExpress Cat# HW-100198 GSK-A1 Sigma Aldrich Cat# SML2453 Critical commercial assays Cholesterol Assay Kit Abcam Cat# Ab122116 LipofectamineTM 2000 Life Technologies Cat# 11668019 LipofectamineTM 3000 Life Technologies Cat# L3000015 LipofectamineTM RNAiMAX Life Technologies Cat# 13778150 NaveniFlex 100 MR Navinci NC.MR.100 Red QuantiTect SYBR Green RT-PCR kit Qiagen Cat# 204443 Deposited Data FIB-SEM dataset of a mouse pancreas OpenOrganelle https://open.quiltdata.com/ b/janelia-cosem-datasets/ tree/jrc_mus-pancreas-3/jrc_ mus-pancreas-3.n5/em/ Experimental models: Cell lines MIN6 cells Yamamura Lab Mazayaki et al.55 Ins-1 cells Alejandra Tomas (Imperial College, UK) N/A (Continued on next page) Cell Reports 43, 113992, April 23, 2024 17
Recombinant:Article Title: OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Calnexin Invitrogen Cat# PA5-34754; RRID: AB_2552106 Chromogranin A Proteintech Cat# 10529-1-AP; RRID: AB_2081122 GolgiB1 Bioss Cat# BS-13356R Insulin Dako Cat# A0564; RRID: AB_10013624 IP3R1 Abcam Cat# ab264281 OSBP Invitrogen Cat# PA5-30110; RRID: AB_2547584 PI4K2A Santa Cruz Biotechnology Cat# sc-390026 Rab3 Synaptic Systems Cat# 107 111 VAPA Atlas Antibodies Cat# HPA009174; RRID: AB_1080549 VAPB Atlas Antibodies Cat# HPA013144; RRID: AB_1858717 b-actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 Bacterial and virus strains Ad-CMV-GB-Rab3 Vector Biolabs Lot# 20220125T#2 Ad-CMV-RA-Sec61b Vector Biolabs Lot# 20220125T#3 Chemicals, peptides, and recombinant proteins BCECF Life technologies Cat# B1170 Cal-520-AM AAT Bioquest Cat# 21130 Cal-590-AM AAT Bioquest Cat# 20510 OSW-1 Cayman Chemical Cat# 30310 TopFluor Cholesterol (23- [dipyrrometheneboron difluoride]24-norcholesterol] Avanti Polar Lipids, Inc. Cat# 810255 Filipin III Cayman Chemical Cat# 70440 JFX650 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JF549 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JFX646 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A Carbamoyl chloride Tocris Cat# 2810 Phenylarsine oxide Sigma Aldrich Cat# P3075 PIK 93 Sigma Aldrich Cat# SML0546 PI4KI MedChemExpress Cat# HW-100198 GSK-A1 Sigma Aldrich Cat# SML2453 Critical commercial assays Cholesterol Assay Kit Abcam Cat# Ab122116 LipofectamineTM 2000 Life Technologies Cat# 11668019 LipofectamineTM 3000 Life Technologies Cat# L3000015 LipofectamineTM RNAiMAX Life Technologies Cat# 13778150 NaveniFlex 100 MR Navinci NC.MR.100 Red QuantiTect SYBR Green RT-PCR kit Qiagen Cat# 204443 Deposited Data FIB-SEM dataset of a mouse pancreas OpenOrganelle https://open.quiltdata.com/ b/janelia-cosem-datasets/ tree/jrc_mus-pancreas-3/jrc_ mus-pancreas-3.n5/em/ Experimental models: Cell lines MIN6 cells Yamamura Lab Mazayaki et al.55 Ins-1 cells Alejandra Tomas (Imperial College, UK) N/A (Continued on next page) Cell Reports 43, 113992, April 23, 2024 17
Cholesterol Assay:Article Title: OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Calnexin Invitrogen Cat# PA5-34754; RRID: AB_2552106 Chromogranin A Proteintech Cat# 10529-1-AP; RRID: AB_2081122 GolgiB1 Bioss Cat# BS-13356R Insulin Dako Cat# A0564; RRID: AB_10013624 IP3R1 Abcam Cat# ab264281 OSBP Invitrogen Cat# PA5-30110; RRID: AB_2547584 PI4K2A Santa Cruz Biotechnology Cat# sc-390026 Rab3 Synaptic Systems Cat# 107 111 VAPA Atlas Antibodies Cat# HPA009174; RRID: AB_1080549 VAPB Atlas Antibodies Cat# HPA013144; RRID: AB_1858717 b-actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 Bacterial and virus strains Ad-CMV-GB-Rab3 Vector Biolabs Lot# 20220125T#2 Ad-CMV-RA-Sec61b Vector Biolabs Lot# 20220125T#3 Chemicals, peptides, and recombinant proteins BCECF Life technologies Cat# B1170 Cal-520-AM AAT Bioquest Cat# 21130 Cal-590-AM AAT Bioquest Cat# 20510 OSW-1 Cayman Chemical Cat# 30310 TopFluor Cholesterol (23- [dipyrrometheneboron difluoride]24-norcholesterol] Avanti Polar Lipids, Inc. Cat# 810255 Filipin III Cayman Chemical Cat# 70440 JFX650 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JF549 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JFX646 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A Carbamoyl chloride Tocris Cat# 2810 Phenylarsine oxide Sigma Aldrich Cat# P3075 PIK 93 Sigma Aldrich Cat# SML0546 PI4KI MedChemExpress Cat# HW-100198 GSK-A1 Sigma Aldrich Cat# SML2453 Critical commercial assays Cholesterol Assay Kit Abcam Cat# Ab122116 LipofectamineTM 2000 Life Technologies Cat# 11668019 LipofectamineTM 3000 Life Technologies Cat# L3000015 LipofectamineTM RNAiMAX Life Technologies Cat# 13778150 NaveniFlex 100 MR Navinci NC.MR.100 Red QuantiTect SYBR Green RT-PCR kit Qiagen Cat# 204443 Deposited Data FIB-SEM dataset of a mouse pancreas OpenOrganelle https://open.quiltdata.com/ b/janelia-cosem-datasets/ tree/jrc_mus-pancreas-3/jrc_ mus-pancreas-3.n5/em/ Experimental models: Cell lines MIN6 cells Yamamura Lab Mazayaki et al.55 Ins-1 cells Alejandra Tomas (Imperial College, UK) N/A (Continued on next page) Cell Reports 43, 113992, April 23, 2024 17
SYBR Green Assay:Article Title: OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Calnexin Invitrogen Cat# PA5-34754; RRID: AB_2552106 Chromogranin A Proteintech Cat# 10529-1-AP; RRID: AB_2081122 GolgiB1 Bioss Cat# BS-13356R Insulin Dako Cat# A0564; RRID: AB_10013624 IP3R1 Abcam Cat# ab264281 OSBP Invitrogen Cat# PA5-30110; RRID: AB_2547584 PI4K2A Santa Cruz Biotechnology Cat# sc-390026 Rab3 Synaptic Systems Cat# 107 111 VAPA Atlas Antibodies Cat# HPA009174; RRID: AB_1080549 VAPB Atlas Antibodies Cat# HPA013144; RRID: AB_1858717 b-actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 Bacterial and virus strains Ad-CMV-GB-Rab3 Vector Biolabs Lot# 20220125T#2 Ad-CMV-RA-Sec61b Vector Biolabs Lot# 20220125T#3 Chemicals, peptides, and recombinant proteins BCECF Life technologies Cat# B1170 Cal-520-AM AAT Bioquest Cat# 21130 Cal-590-AM AAT Bioquest Cat# 20510 OSW-1 Cayman Chemical Cat# 30310 TopFluor Cholesterol (23- [dipyrrometheneboron difluoride]24-norcholesterol] Avanti Polar Lipids, Inc. Cat# 810255 Filipin III Cayman Chemical Cat# 70440 JFX650 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JF549 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JFX646 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A Carbamoyl chloride Tocris Cat# 2810 Phenylarsine oxide Sigma Aldrich Cat# P3075 PIK 93 Sigma Aldrich Cat# SML0546 PI4KI MedChemExpress Cat# HW-100198 GSK-A1 Sigma Aldrich Cat# SML2453 Critical commercial assays Cholesterol Assay Kit Abcam Cat# Ab122116 LipofectamineTM 2000 Life Technologies Cat# 11668019 LipofectamineTM 3000 Life Technologies Cat# L3000015 LipofectamineTM RNAiMAX Life Technologies Cat# 13778150 NaveniFlex 100 MR Navinci NC.MR.100 Red QuantiTect SYBR Green RT-PCR kit Qiagen Cat# 204443 Deposited Data FIB-SEM dataset of a mouse pancreas OpenOrganelle https://open.quiltdata.com/ b/janelia-cosem-datasets/ tree/jrc_mus-pancreas-3/jrc_ mus-pancreas-3.n5/em/ Experimental models: Cell lines MIN6 cells Yamamura Lab Mazayaki et al.55 Ins-1 cells Alejandra Tomas (Imperial College, UK) N/A (Continued on next page) Cell Reports 43, 113992, April 23, 2024 17
Reverse Transcription Polymerase Chain Reaction:Article Title: OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Calnexin Invitrogen Cat# PA5-34754; RRID: AB_2552106 Chromogranin A Proteintech Cat# 10529-1-AP; RRID: AB_2081122 GolgiB1 Bioss Cat# BS-13356R Insulin Dako Cat# A0564; RRID: AB_10013624 IP3R1 Abcam Cat# ab264281 OSBP Invitrogen Cat# PA5-30110; RRID: AB_2547584 PI4K2A Santa Cruz Biotechnology Cat# sc-390026 Rab3 Synaptic Systems Cat# 107 111 VAPA Atlas Antibodies Cat# HPA009174; RRID: AB_1080549 VAPB Atlas Antibodies Cat# HPA013144; RRID: AB_1858717 b-actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 Bacterial and virus strains Ad-CMV-GB-Rab3 Vector Biolabs Lot# 20220125T#2 Ad-CMV-RA-Sec61b Vector Biolabs Lot# 20220125T#3 Chemicals, peptides, and recombinant proteins BCECF Life technologies Cat# B1170 Cal-520-AM AAT Bioquest Cat# 21130 Cal-590-AM AAT Bioquest Cat# 20510 OSW-1 Cayman Chemical Cat# 30310 TopFluor Cholesterol (23- [dipyrrometheneboron difluoride]24-norcholesterol] Avanti Polar Lipids, Inc. Cat# 810255 Filipin III Cayman Chemical Cat# 70440 JFX650 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JF549 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A JFX646 Halotag Ligand Lavis Lab (Janalia Research Campus) N/A Carbamoyl chloride Tocris Cat# 2810 Phenylarsine oxide Sigma Aldrich Cat# P3075 PIK 93 Sigma Aldrich Cat# SML0546 PI4KI MedChemExpress Cat# HW-100198 GSK-A1 Sigma Aldrich Cat# SML2453 Critical commercial assays Cholesterol Assay Kit Abcam Cat# Ab122116 LipofectamineTM 2000 Life Technologies Cat# 11668019 LipofectamineTM 3000 Life Technologies Cat# L3000015 LipofectamineTM RNAiMAX Life Technologies Cat# 13778150 NaveniFlex 100 MR Navinci NC.MR.100 Red QuantiTect SYBR Green RT-PCR kit Qiagen Cat# 204443 Deposited Data FIB-SEM dataset of a mouse pancreas OpenOrganelle https://open.quiltdata.com/ b/janelia-cosem-datasets/ tree/jrc_mus-pancreas-3/jrc_ mus-pancreas-3.n5/em/ Experimental models: Cell lines MIN6 cells Yamamura Lab Mazayaki et al.55 Ins-1 cells Alejandra Tomas (Imperial College, UK) N/A (Continued on next page) Cell Reports 43, 113992, April 23, 2024 17
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